Biopanda - Model ELISA-AFB1-001 - Aflatoxin B1 ELISA Kit

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of Aflatoxin B₁. An unknown amount of Aflatoxin B₁ present in the sample and the fixed amount of Aflatoxin B₁ antigens pre-coated on the wells of microtiter plate/strips compete for the anti-Aflatoxin B₁ antibodies, which in turn is detected with enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The absorbance value of the developed colour is inversely proportional to the amount of the Aflatoxin B₁ in the sample. The quantity of Aflatoxin B₁ in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

• 1 x Pre-coated Microtiter plate (12 x 8 microwells)
• 1 x Aflatoxin B₁ Enzyme conjugate (6 ml)
• 1 x Aflatoxin B₁ Antibody solution (6 ml)
• 1 x Wash buffer concentrate (10×, 50 ml)
• 1 x Sample Diluent concentrate (10×, 20 ml)
• 6 x Aflatoxin B₁ standards (1.0 ml/each) (0 ng/ml, 0.05 ng/ml, 0.15 ng/ml, 0.45 ng/ml, 1.35 ng/ml, 4.05 ng/ml)
• 1 x TMB solution (12 ml)
• 1 x Stop solution (14 ml)
• 1 x Package insert
• 1 x Microplate sealer

Storage Conditions

+ 2 - 8 °C

Shelf life

12 months